The product enables rapid and high-quality DNA preparation from blood.

Features

• Chaotropic salt-based silica membrane Purification.
• Purification systems for low throughput approaches.
• Easy procedure for small scale.

Binding

DNA/RNA is interacting to the silica membrane under high salt conditions.

Washing

Contaminants are washed away under conditions that ensure DNA/RNA remain attached to the membrane.

Elution

Highly pure DNA/RNA is eluted under low ionic strength conditions or in pure water. DNA/RNA is ready to use for downstream applications.

Technical Data

Title	Value
Catalogue number	E-1-B
Application	Isolation of DNA
CE certified	Research use only (RUO)
Technology	Silica membrane technology
Brand	DBG-Spin™
Format	Mini prep
Package unit	50 Preps
Handling/Automated use	Centrifugation/No
Sample material	Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum
Fragment size	200 bp–approx. 50 kbp
Typical yield/ Concenteration	4–6 µg/ 40–100 ng/µL
Elution volume	60–200 µL
Preparation time	30 min/prep
Downstream application	Enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting
Storage temperature	15−25 °C
IFU	Download

“One extraction system to rule them all”.

DBG TL™ Lysis Buffer when combined with DBG-Spin™ Blood DNA Kit allows for the extraction of DNA from a variety of samples, and enables various applications:

1. Mouse or rat tail
2. Bacteria
3. Hair roots
4. Dried blood spots (FTA cards, Guthrie cards)
5. Yeast
6. Blood samples
7. Paraffin embedded tissue
8. Stool (genomic DNA)
9. Stool (viral DNA)
10. Sputum or bronchoalveolar lavage (for Mycobacterium tuberculosis or Legionella pneumophila)
11. Food (e.g. fresh cows’ milk for detection of EHEC bacteria)
12. Cultures, biological fluids or clinical specimens (bacterial DNA)
13. Urine (for bacterial DNA e.g. Borrelia burgdorferi)
14. Urine (viral DNA)
15. Insects
16. Dental swabs
17. Semen
18. Buccal swabs
19. Cyanobacteria
20. Bacterial DNA for MRSA

The obvious benefit of the combination of DBG TL™ Lysis Buffer and DBG-Spin™ Blood DNA Kit is the high versatility which can be translated into cost-effectiveness. This advantage together with the easy set-up (robustness) of the system makes this combination an ideal solution for forensic-, clinical laboratories, and research institutes.

Technical Data

Title	Value
Catalogue number	A-3-B.20
Application	Extraction of DNA from a variety of sample material
CE certified	Research use only (RUO)
Format	Solution
Package unit	20 ml
Downstream application	Enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting
Storage temperature	15−25 °C
IFU	Protocol for bacteria Protocol for detection of EHEC bacteria in food (e.g., fresh cows’ milk) Protocol for dried blood spots Protocol for genomic DNA and viral DNA from blood samples Protocol for genomic DNA from stool Protocol for hair roots Protocol for Methicillin-resistant Staphylococcus aureus (MRSA) Protocol for mouse or rat tails Protocol for Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage Protocol for paraffin-embedded tissue Protocol for purification of bacterial DNA (e.g., Chlamydia trachomatis) from cultures biological fluids or clinical specimens Protocol for purification of bacterial DNA (e.g.,Borrelia burgdorferi) from urine Protocol for purification of genomic DNA from cyanobacteria Protocol for purification of genomic DNA from dental swabs Protocol for purification of genomic DNA from insects Protocol for purification of genomic DNA from semen Protocol for purification of genomic DNA from buccal swabs Protocol for purification of viral DNA (e.g., CMV) from urine Protocol for viral DNA (e.g., CMV) from stool Protocol for yeast Standard protocol for human or animal tissue

Rapid and high-quality DNA preparation from virus.

Features:

• Chaotropic salt-based silica membrane purification.
• Purification systems for low throughput approaches.
• Easy procedure for small scale.

Binding

DNA/RNA is interacting to the silica membrane under high salt conditions.

Washing

Contaminants are washed away under conditions that ensure DNA/RNA remain attached to the membrane.

Elution

Highly pure DNA/RNA is eluted under low ionic strength conditions or in pure water. DNA/RNA is ready to use for downstream applications.

Technical Data

Title	Value
Catalogue number	E-2-V
Application	Isolation of viral nucleic acids
CE certified	Research use only (RUO)
Technology	Silica membrane technology
Brand	DBG-Spin
Format	Mini prep
Package unit	50 Preps
Handling/ Automated use	Centrifugation/No
Sample material	Cell-free biological fluid, Plasma, Serum
Fragment size	100 bp−approx. 50 kbp
Theoretical binding capacity	25 µg
Elution volume	30 µL
Preparation time	50 min/6 preps
Downstream application	Enzymatic reactions, PCR, RT-PCR
Storage temperature	15−25 °C
IFU	Download